URT1
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The TUTase URT1 connects decapping activators and prevents the ...
Feb 26, 2021 a Domain organization and mutations of URT1-myc versions. b Western blot analysis of URT1-myc expression. c–e Distribution profiles of GFP mRNA poly(A) tail sizes. …See details»
Crystal structure of Arabidopsis terminal uridylyl transferase URT1
Apr 2, 2020 The crystal structure of URT1 enzymatic domain. (A) Schematic representation of the domain organization of URT1. The C-terminal region 425–756 of URT1 contains a …See details»
RCSB PDB - 6L3F: The structure of UTP:RNA uridylyltransferase 1 …
Oct 10, 2019 Moreover, URT1 N552 may be an important residue in interacting with 3' nucleotide of RNA substrate. The URT1 structure we determined represents the first structure …See details»
Uridylation Earmarks mRNAs for Degradation… and More
Oct 1, 2016 Domain Organization of Terminal Uridylyltransferases (TUTases) Uridylating mRNAs. Nucleotidyltransferase domain (NTD) is shown in red, poly(A) polymerase-associated …See details»
The TUTase URT1 connects decapping activators and prevents the ...
Feb 26, 2021 Ectopic expression of URT1 remodels poly(A) tail profiles a Domain organization and mutations of different URT1-myc versions transiently expressed in N. benthamiana leaf …See details»
Molecular mechanism underlying the di-uridylation …
Sep 30, 2022 At the +1 position, the terminal U (U +1) of RNA stretch adopted very similar conformations for its base and sugar ring as those of the UTP molecule in the URT1-UTP structure (PDB ID 6L8K) (Supplementary Figure …See details»
The TUTase URT1 connects decapping activators and prevents the ...
Interaction connects URT1 to additional decay factors like DDX6/Dhh1-like RNA helicases. Nanopore direct RNA sequencing reveals a global role of URT1 in shaping poly(A) tail length, …See details»
7XS4: Crystal structure of URT1 in complex with AAAU …
May 12, 2022 We previously solved the three-dimensional structure of URT1 in the absence and presence of UTP. In this study, we further determined the structure of URT1 in complex with a 5'-AAAU-3' RNA stretch that mimics the …See details»
Crystal structure of Arabidopsis terminal uridylyl transferase URT1
Apr 2, 2020 3' uridylation is an essential modification associated with coding and noncoding RNA degradation in eukaryotes. In Arabidopsis, HESO1 was first identified as the major …See details»
The TUTase URT1 connects decapping activators and prevents the ...
Feb 26, 2021 URT1 directly interacts with DECAPPING 5 (DCP5), the Arabidopsis ortholog of human LSM14 and yeast Scd6, and this interaction connects URT1 to additional decay factors …See details»
Crystal structure of Arabidopsis terminal uridylyl transferase URT1 ...
Apr 2, 2020 The URT1 structure we determined represents the first structure of uridylyl transferase from plants, shedding light on the mechanisms of URT1/HESO1-dependent RNA …See details»
Respective Contributions of URT1 and HESO1 to the Uridylation of …
Two replicates are shown in black and orange, respectively, for WT, urt1-1, the urt1 SIL line, heso1-1, and the three heso1-1 urt1 SIL lines (i.e., six heso1-1 urt1 SIL samples). (C) …See details»
Accumulation of oligo(A) uridylated and A-rich-tailed GFP mRNAs …
Download scientific diagram | Accumulation of oligo(A) uridylated and A-rich-tailed GFP mRNAs upon overexpression of URT1D491/3A requires the M1 motif a Domain organization and …See details»
Distinct and Cooperative Activities of HESO1 and URT1 ... - PLOS
Apr 30, 2015 pURT1:URT1-GFP1 and pURT1:URT1-GFP2 are two independent T1 lines of hen1-8 urt1-1 containing the transgene. (B) The heso1-1 or urt1-1 mutation does not affect the …See details»
6L8K: Structure of URT1 in complex with UTP - RCSB PDB
Moreover, URT1 N552 may be an important residue in interacting with 3' nucleotide of RNA substrate. The URT1 structure we determined represents the first structure of uridylyl …See details»
O64642 - UniProt
Jun 1, 2002 URT1 and HESO1 act sequentially, with URT1 mono-uridylating the miRNAs followed by their further uridylation by HESO1 (PubMed:25928405). URT1 and HESO1 are …See details»
The TUTase URT1 connects decapping activators and prevents the ...
A Domain organization and mutations of URT1-myc versions. b Western blot analysis of URT1-myc expression. c–e Distribution profiles of GFP mRNA poly(A) tail sizes. The percentages of …See details»
Molecular mechanism underlying the di-uridylation activity of ...
Oct 14, 2022 In this study, we further determined the structure of URT1 in complex with a 5'-AAAU-3' RNA stretch that mimics the post-catalytic state of the mRNA poly(A) tail after the …See details»
Distinct and cooperative activities of HESO1 and URT1 nucleotidyl ...
Apr 30, 2015 URT1 and HESO1 prefer substrates with different 3' end nucleotides in vitro and act cooperatively to tail different forms of the same miRNAs in vivo. Moreover, both HESO1 …See details»