URT1

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The TUTase URT1 connects decapping activators and prevents the ...

Feb 26, 2021 a Domain organization and mutations of different URT1-myc versions transiently expressed in N. benthamiana leaf patches. Legend as in Fig. 1a . b Western blot analysis of …See details»

Crystal structure of Arabidopsis terminal uridylyl transferase URT1

Apr 2, 2020 The crystal structure of URT1 enzymatic domain. (A) Schematic representation of the domain organization of URT1. The C-terminal region 425–756 of URT1 contains a …See details»

RCSB PDB - 6L3F: The structure of UTP:RNA …

Oct 10, 2019 Moreover, URT1 N552 may be an important residue in interacting with 3' nucleotide of RNA substrate. The URT1 structure we determined represents the first structure of uridylyl transferase from plants, shedding light …See details»

The TUTase URT1 connects decapping activators and prevents the ...

Fig. 1 Short linear motifs are conserved in plant URT1 orthologs. a Domain organization of URT1. CCD catalytic core domain, Ntrf polymerase β-like nucleotidyltransferase domain, PAP-assoc …See details»

The TUTase URT1 connects decapping activators and prevents the ...

Feb 26, 2021 URT1 directly interacts with DECAPPING 5 (DCP5), the Arabidopsis ortholog of human LSM14 and yeast Scd6, and this interaction connects URT1 to additional decay factors …See details»

7XS4: Crystal structure of URT1 in complex with AAAU …

May 12, 2022 We previously solved the three-dimensional structure of URT1 in the absence and presence of UTP. In this study, we further determined the structure of URT1 in complex with a 5'-AAAU-3' RNA stretch that mimics the …See details»

The TUTase URT1 connects decapping activators and prevents the ...

Feb 26, 2021 a Domain organization and mutations of URT1-myc versions. b Western blot analysis of URT1-myc expression. c–e Distribution profiles of GFP mRNA poly(A) tail sizes. …See details»

Crystal structure of Arabidopsis terminal uridylyl transferase URT1

Apr 2, 2020 3' uridylation is an essential modification associated with coding and noncoding RNA degradation in eukaryotes. In Arabidopsis, HESO1 was first identified as the major …See details»

Molecular connection between the TUTase URT1 and decapping

May 28, 2020 Uridylation is a widespread modification destabilizing eukaryotic mRNAs. Yet, molecular mechanisms underlying TUTase-mediated mRNA degradation remain mostly …See details»

RCSB PDB - 6L8K: Structure of URT1 in complex with UTP

Nov 6, 2019 Moreover, URT1 N552 may be an important residue in interacting with 3' nucleotide of RNA substrate. The URT1 structure we determined represents the first structure of uridylyl transferase from plants, shedding light …See details»

Distinct and Cooperative Activities of HESO1 and URT1 …

A .gov website belongs to an official government organization in the United States. Secure .gov websites use HTTPS A lock ... When a URT1-GFP transgene driven by the URT1 promoter was introduced into hen1-8 urt1-1, the increase …See details»

O64642 - UniProt

Jun 1, 2002 URT1 and HESO1 act sequentially, with URT1 mono-uridylating the miRNAs followed by their further uridylation by HESO1 (PubMed:25928405). URT1 and HESO1 are …See details»

The TUTase URT1 regulates the transcriptome of seeds and

Dec 21, 2024 Our transcriptome-wide analysis showed that, URT1 is the main enzyme responsible for mRNA uridylation, in all analyzed tissues, while HESO1 can marginally …See details»

Crystal structure of Arabidopsis terminal uridylyl transferase URT1 ...

Apr 2, 2020 The URT1 structure we determined represents the first structure of uridylyl transferase from plants, shedding light on the mechanisms of URT1/HESO1-dependent RNA …See details»

The TUTase URT1 connects decapping activators and prevents the ...

A Domain organization and mutations of URT1-myc versions. b Western blot analysis of URT1-myc expression. c–e Distribution profiles of GFP mRNA poly(A) tail sizes. The percentages of …See details»

Mechanism of U6 snRNA oligouridylation by human TUT1

Aug 10, 2023 This preference enables URT1 to control the optimal number of uridines and URT1 added two uridines to the 3′ extremity of polyA. L527 and Y592 of AtURT1 corresponds …See details»

Molecular mechanism underlying the di-uridylation activity of ...

Oct 14, 2022 In this study, we further determined the structure of URT1 in complex with a 5'-AAAU-3' RNA stretch that mimics the post-catalytic state of the mRNA poly(A) tail after the …See details»

The TUTase URT1 regulates the transcriptome of seeds and

Dec 21, 2024 respective contribution of the two known Arabidopsis uridylyltransferases, URT1 and HESO1, in mRNA uridylation. Our transcriptome-wide analysis showed that, URT1 is the …See details»

The TUTase URT1 connects decapping activators and prevents the ...

Feb 26, 2021 Ectopic expression of URT1 remodels poly(A) tail profiles a Domain organization and mutations of different URT1-myc versions transiently expressed in N. benthamiana leaf …See details»

Distinct and cooperative activities of HESO1 and URT1 nucleotidyl ...

Apr 30, 2015 URT1 and HESO1 prefer substrates with different 3' end nucleotides in vitro and act cooperatively to tail different forms of the same miRNAs in vivo. Moreover, both HESO1 …See details»